An acute bout of high-intensity interval training increases the nuclear abundance of PGC-1α and activates mitochondrial biogenesis in human skeletal muscle.

@article{Little2011AnAB,
  title={An acute bout of high-intensity interval training increases the nuclear abundance of PGC-1$\alpha$ and activates mitochondrial biogenesis in human skeletal muscle.},
  author={Jonathan Peter Little and Adeel Safdar and David J. Bishop and Mark Andrew Tarnopolsky and Martin J. Gibala},
  journal={American journal of physiology. Regulatory, integrative and comparative physiology},
  year={2011},
  volume={300 6},
  pages={
          R1303-10
        }
}
  • J. LittleA. Safdar M. Gibala
  • Published 30 March 2011
  • Biology
  • American journal of physiology. Regulatory, integrative and comparative physiology
Low-volume, high-intensity interval training (HIT) increases skeletal muscle mitochondrial capacity, yet little is known regarding potential mechanisms promoting this adaptive response. Our purpose was to examine molecular processes involved in mitochondrial biogenesis in human skeletal muscle in response to an acute bout of HIT. Eight healthy men performed 4 × 30-s bursts of all-out maximal intensity cycling interspersed with 4 min of rest. Muscle biopsy samples (vastus lateralis) were… 
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Highly Influential Citations
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329 Citations

Forty high-intensity interval training sessions blunt exercise-induced changes in the nuclear protein content of PGC-1α and p53 in human skeletal muscle.

Forty sessions of twice-daily high-intensity interval training blunted all of the measured exercise-induced molecular events associated with mitochondrial biogenesis that were observed Pre-HVT.

Forty high-intensity interval training sessions blunt exercise-induced changes in the nuclear protein content of PGC-1α and p53 in human skeletal muscle

Forty sessions of twice-daily high-intensity interval training blunted all of the measured exercise-induced molecular events associated with mitochondrial biogenesis that were observed Pre-HVT.

Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training

Over only 2 weeks HIT significantly increased mitochondrial function in skeletal muscle independently of detectable changes in mitochondrial-associated and mitogenic protein expression.

Rapidly elevated levels of PGC-1α-b protein in human skeletal muscle after exercise: exploring regulatory factors in a randomized controlled trial.

The present study contributes new insights into the initial signaling events following a single bout of exercise and emphasizes PGC-1α-ex1b as the most exercise-responsive P GC-1 α isoform.

Sprint-interval but not continuous exercise increases PGC-1α protein content and p53 phosphorylation in nuclear fractions of human skeletal muscle

Increased nuclear p53 phosphorylation and PGC-1α protein content immediately following SIE but not CE suggests these may represent important early molecular events in the exercise-induced response to exercise, and that SIE is a time-efficient and possibly superior option to promote these adaptations.

A systematic upregulation of nuclear and mitochondrial genes is not present in the initial postexercise recovery period in human skeletal muscle.

The lack of observed systematic upregulation of nuclear- and mitochondrial-encoded genes suggests that exercise-induced up regulation of PGC-1α targets are differentially regulated during the initial hours following acute exercise in humans.

Short-Term Intensified Cycle Training Alters Acute and Chronic Responses of PGC1α and Cytochrome C Oxidase IV to Exercise in Human Skeletal Muscle

It is demonstrated that short-term intensified training promotes increased mitochondrial gene expression and protein abundance and acute indicators of exercise-induced mitochondrial adaptation appear to be blunted in response to exercise at the same absolute intensity following short- term training.

High-intensity interval training increases in vivo oxidative capacity with no effect on P(i)→ATP rate in resting human muscle.

This novel analysis of resting and maximal high-energy phosphate kinetics in vivo in response to HIT provides evidence that distinct aspects of human skeletal muscle metabolism respond differently to this type of training.

Exercise twice-a-day potentiates markers of mitochondrial biogenesis in men

The results suggest that part of the elevated molecular signalling reported with previous “train-low” approaches might be attributed to performing two exercise sessions in close proximity and the twice-a-day approach might be an effective strategy to induce adaptations related to mitochondrial biogenesis and fat oxidation.

Attenuated PGC-1α Isoforms following Endurance Exercise with Blood Flow Restriction.

The attenuated expression of all four PGC-1α isoforms when EE is performed with blood flow restriction suggests this type of exercise provides an insufficient stimulus to activate the signaling pathways governing mitochondrial and angiogenesis responses observed with moderate- to high-intensity EE.
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