An acrosomal protein, sp32, in mammalian sperm is a binding protein specific for two proacrosins and an acrosin intermediate.

@article{Baba1994AnAP,
  title={An acrosomal protein, sp32, in mammalian sperm is a binding protein specific for two proacrosins and an acrosin intermediate.},
  author={Tadashi Baba and Yo Niida and Yu-ichi Michikawa and Shin-ichi Kashiwabara and Kunihiko Kodaira and Mio Takenaka and Nobuoki Kohno and George L. Gerton and Yoshikazu Arai},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 13},
  pages={10133-40}
}
An acrosomal protein, sp32, was completely purified from acid extracts of ejaculated porcine sperm. Purified sp32 gave a single 32-kDa protein band on SDS-polyacrylamide gel electrophoresis and was characterized as a binding protein specific for 55-, 53-, and 49-kDa forms of (pro)acrosin. This protein was not capable of binding a 43-kDa acrosin intermediate and 35-kDa mature acrosin. sp32 significantly accelerated autoactivation of proacrosin at a basic pH in vitro and affected the maturation… CONTINUE READING