An acetylcholinesterase method for in toto staining of peripheral nerves.

@article{Baljet1975AnAM,
  title={An acetylcholinesterase method for in toto staining of peripheral nerves.},
  author={Bob Baljet and Jan Drukker},
  journal={Stain technology},
  year={1975},
  volume={50 1},
  pages={31-6}
}
UNLABELLED Stomach, small intestine, uterus, urinary bladder, vagina, mesentery, mesometrium and joint capsule of rats, gall bladder, cystic duct and bile duct of dogs and uteri of young children are stained in toto. PROCEDURE Tissue is perfused with saline containing hyaluronidase, then pinned on a flat layer of Paraplast and fixed for 24 hr in cold sucrose formol solution. Stomach, urinary bladder and gall bladder are also fixed in toto. Rinse for 2 days in cold 0.22 M sucrose in a sodium… CONTINUE READING

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