[An XcmⅠ-generated T vector and its applications in synthetic biology].

Abstract

For more economical and efficient DNA clonging, pFL-XS-T, a Biobrick-T vector was constructed based on pMD18-T vector, carrying clonging regions of XbaⅠ-XcmⅠ-XcmⅠ-SpeⅠ. The results revealed that PCR products could be conveniently inserted into pFL-XS-T vevtor digested by XcmⅠby means of TA cloning. The positive frequency of recombination can meet the experimental requirements and all the plasmids obtained meet Biobrick standard. Moreover, the pFL-XS-T is compatible with other Biobrick parts, and serves as a vector for functional DNA fragments screening.

DOI: 10.13345/j.cjb.150448

Cite this paper

@article{Duan2016AnXT, title={[An XcmⅠ-generated T vector and its applications in synthetic biology].}, author={Yangkai Duan and Feiyan Liang and Xiaoming Tan and Xuefeng Lv}, journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology}, year={2016}, volume={32 7}, pages={956-965} }