An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus.

@article{Prez2012AnSG,
  title={An SYBR Green-based real-time RT-PCR assay for the detection of H5 hemagglutinin subtype avian influenza virus.},
  author={Lester J P{\'e}rez and Heidy D{\'i}az de Arce and Filippo Cilloni and Annalisa Salviato and Sabrina Marciano and Carmen Laura Perera and Angela Salomoni and Maria Serena Beato and Antonio Romero and Ilaria 3 Capua and Giovanni Cattoli},
  journal={Molecular and cellular probes},
  year={2012},
  volume={26 3},
  pages={137-45}
}
Increasing diversity among H5 hemagglutinin (HA) subtype avian influenza (AI) viruses has resulted in the need of novel sensitive and specific molecular assays. In this study, an SYBR Green-based real-time reverse transcription-PCR (RRT-PCR) assay was developed for the detection of H5 subtype AI virus. Sequence analysis of the Mexican lineage H5N2 isolates (subgroup B) revealed several mismatches in the primer/hydrolysis probe set reported in the commonly used RRT-PCR assay for the detection of… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 38 references

Evaluation of Primer and Probe Mismatches in Sensitivity of Select RRT-PCR Tests; 2010. In: OFFLU Technical Meeting. Food and Agriculture Organization of the United Nations, Rome, 16e17 November

DL Suarez
2010
View 1 Excerpt

OFFLU teleconference - laboratory diagnosis of new A/H1N1 in swine

S. Edwards
2009
View 1 Excerpt

Avian in fl uenza

I Capua, DJ Alexander
2008

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