An SV40 large T antigen binding site in the cellular genome is part of a cis-acting transcriptional element.

A genomic mouse DNA fragment (Q300), containing a high affinity binding site for SV40 large T antigen, serves as a cis-acting transcriptional element in in vivo and in in vitro studies. We have performed experiments to investigate whether bound T antigen could modulate the promoter-enhancer activity of the Q300 element. In vivo studies showed a negative… CONTINUE READING