An STS-based map of the human genome.

Abstract

A physical map has been constructed of the human genome containing 15,086 sequence-tagged sites (STSs), with an average spacing of 199 kilobases. The project involved assembly of a radiation hybrid map of the human genome containing 6193 loci and incorporated a genetic linkage map of the human genome containing 5264 loci. This information was combined with the results of STS-content screening of 10,850 loci against a yeast artificial chromosome library to produce an integrated map, anchored by the radiation hybrid and genetic maps. The map provides radiation hybrid coverage of 99 percent and physical coverage of 94 percent of the human genome. The map also represents an early step in an international project to generate a transcript map of the human genome, with more than 3235 expressed sequences localized. The STSs in the map provide a scaffold for initiating large-scale sequencing of the human genome.

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@article{Hudson1995AnSM, title={An STS-based map of the human genome.}, author={Tom Hudson and Lincoln Stein and Sebastian S Gerety and J Ma and Andrew B. Castle and Jos{\'e} Manuel Silva and Donna K. Slonim and Raymundo Baptista and L Kruglyak and S-Y Xu and X Hu and Alison M Colbert and Charles Rosenberg and M P Reeve-Daly and Steven G Rozen and Li-Wei Hui and X Wu and Christian L Vestergaard and Kiesha M. Wilson and Jin Soo Bae and Soumen Maitra and Soula Ganiatsas and Carla A Evans and Matthew deAngelis and Kimberly A Ingalls and Robert Nahf and Lori Horton and Matthew O. Anderson and Alville Collymore and Wei Ye and V Kouyoumjian and I S Zemsteva and Jorge Tam and Randi Devine and DG Courtney and Michelle T Renaud and Huy Van Nguyen and Tamara J O'Connor and C{\'e}cile Fizames and Sabine Faur{\'e} and G{\'a}bor Gyapay and Claudio Dib and Jean Morissette and James B. Orlin and Bruce Birren and Nathan Goodman and Jean Weissenbach and Trevor L Hawkins and Simon J Foote and Don W Cleveland and Eric S. Lander}, journal={Science}, year={1995}, volume={270 5244}, pages={1945-54} }