An RP-HPLC determination of 5-hydroxymethylfurfural in honey The case of strawberry tree honey.

  title={An RP-HPLC determination of 5-hydroxymethylfurfural in honey The case of strawberry tree honey.},
  author={Nadia Spano and Lucia Casula and Angelo Panzanelli and Maria Itria Pilo and Paola Costantina Piu and Roberta Scanu and Andrea Tapparo and Gavino Sanna},
  volume={68 4},
The use of the RP-HPLC official method of the International Honey Commission (IHC) for the determination of 5-hydroxymethylfurfural (HMF) in strawberry tree honey (Arbutus unedo, a typical Sardinian honey) has brought to light a specific and heavy chromatographic interference that prevents accurate quantification. The interference has been identified as homogentisic acid (HA), i.e. the marker of the botanical origin of the honey. For this reason, an alternative RP-HPLC method is proposed. The… Expand
A direct RP-HPLC method for the determination of furanic aldehydes and acids in honey.
Honey samples of different ages, and botanical and geographical origin, correlated highly with the age of the samples, whereas no correlation was observed with regards to 2-furaldehyde and 2- furoic acid. Expand
Determination of 5-hydroxymethyl-2-furaldehyde in royal jelly by a rapid reversed phase HPLC method
A new method for the determination of 5-hydroxymethyl-2-furaldehyde (HMF) in royal jelly by reversed phase HPLC is presented. The method is rapid and simple and it has been validated in terms ofExpand
Survey of 1,2-dicarbonyl compounds in commercial honey of different floral origin.
The chemical characteristics that most influenced the levels of 1,2-dicarbonyl compounds in honey were found to be pH and total phenols and were supported by multivariate analysis used to classify different honey types with respect to their chemical characteristics. Expand
Determination of very low levels of 5-(hydroxymethyl)-2-furaldehyde (HMF) in natural honey: comparison between the HPLC technique and the spectrophotometric white method.
The HPLC method seems to be more appropriate for the determination of HMF in honey in the range 1-4 mg/kg thanks to its greater precision, but for samples with a HMF content of less than 1mg/kg the analyses are inaccurate for both methods. Expand
Fast quantitation of 5-hydroxymethylfurfural in honey using planar chromatography
It was demonstrated that the proposed HPTLC method is an effective method for HMF quantitation in honey at the strictest regulated level of 15 mg kg−1. Expand
Determination and Safety Evaluation of Furfural and Hydroxymethylfurfural in Some Honey Samples by Using a Validated HPLC-DAD Method
A novel high performance liquid chromatography method has been developed and validated for the simultaneous determination of furfural and hydroxymethylfurfural in 18 honey samples. An AgilentExpand
High 5-hydroxymethylfurfural concentrations are found in Malaysian honey samples stored for more than one year.
  • M. I. Khalil, S. A. Sulaiman, S. Gan
  • Chemistry, Medicine
  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2010
It is recommended that honey should generally be consumed within one year, regardless of the type, and physicochemical properties of honey showed significant correlation with HMF content and may provide parameters that could be used to make quick assessments of honey quality. Expand
The quantitation of hydroxymethylfurfural in Australian Leptospermum honeys
This research extends the rapid high-performance liquid chromatographic (HPLC) analysis of O-(2, 3, 4, 5, 6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) derivatives of methylglyoxal andExpand
A comparison of two methods for determination of HMF in honey and bee food : HPLC method versus spectrophotometric Winkler method
Hydroxymethylfurfural (HMF) is an organic compound resulting from the dehydration of certain sugars. Elevated concentrations of HMF are an indicator of overheating, storage in poor conditions and ageExpand
RP-HPLC determination of water-soluble vitamins in honey.
This study proposes and fully validates a simple and fast RP-HPLC method for the simultaneous determination of five water-soluble vitamins in honey and observes a marked dependence of some of their concentrations and the botanical origin of the honey. Expand


Direct chromatographic methods for the rapid determination of homogentisic acid in strawberry tree (Arbutus unedo L.) honey.
Two rapid and direct chromatographic methods based on reverse phase-high performance liquid chromatography (RP-HPLC) and ion chromatography (IC) were developed for the determination of homogentisicExpand
A review of the analytical methods to determine the geographical and botanical origin of honey
Abstract This review is concerned with analytical methods to prove the authenticity of honey. A special emphasis is put on suitable methods for the detection of the geographical and botanical originExpand
Effects of conditioning on HMF content in unifloral honeys
Abstract HMF (5-hydroxymethylfurfural) is considered an important quality parameter for honey and maximum values are fixed by the European Directive no.110 (2001). In this paper, the HMF levelsExpand
Homogentisic acid: a phenolic acid as a marker of strawberry-tree (Arbutus unedo) honey.
Analysis of organic acids in strawberry-tree (Arbutus unedo) honey showed the presence of an unknown acid as the main constituent. This compound was isolated and identified as homogentisic acid (2,Expand
Valutazione preliminare sulla capacity antiradicalica in vitro e sul contenuto in polifenoli di mieli di diversa origine floreale
Honey is a food source of natural components which antioxidant power is comparable to that one of many fruits and vegetables. Honey has been used in place of antioxidant products because of itsExpand
Solid-phase extraction and high-performance liquid chromatographic determination of organic acids in honey
Abstract A high-performance liquid chromatographic method is reported that allows the determination of organic acids in honey after sample purification by solid-phase extraction. The chromatographicExpand
5-Hydroxymethylfurfural: assessment of mutagenicity, DNA-damaging potential and reactivity towards cellular glutathione.
The results suggest that HMF does not pose a serious health risk, even though the highest concentrations in specific foods approach the biologically effective concentration range in cell systems. Expand
5-Sulfooxymethylfurfural as a possible ultimate mutagenic and carcinogenic metabolite of the Maillard reaction product, 5-hydroxymethylfurfural.
5-Chloromethylfurfural was found to be a strong hepatocarcinogen in infant male B6C3F1 mice and induced dose-dependent increases in the number of His+ revertants in Salmonella typhimurium TA100. Expand
Initiation and promotion of colonic aberrant crypt foci in rats by 5-hydroxymethyl-2-furaldehyde in thermolyzed sucrose.
It is concluded that sugar heated under household cooking conditions may act as both an initiator and a promoter of colon cancer because of the presence of HMF. Expand
Nomenclature for the presentation of results of chemical analysis (IUPAC Recommendations 1994)
Chairman: G. Svehla (UK), Secretary: S . P. Perone (USA). Titular Members: C. A. M. G. Cramers (Netherlands), R. W. Frei (Netherlands), R. E. van Grieken (Belgium), D. Klockow (FRG). AssociateExpand