An RNAi system in Physcomitrella patens with an internal marker for silencing allows for rapid identification of loss of function phenotypes.

@article{Bezanilla2005AnRS,
  title={An RNAi system in Physcomitrella patens with an internal marker for silencing allows for rapid identification of loss of function phenotypes.},
  author={Magdalena Bezanilla and Pierre-François Perroud and Aihu Pan and Paul Klueh and Ralph S. Quatrano},
  journal={Plant biology},
  year={2005},
  volume={7 3},
  pages={251-7}
}
RNAi is a powerful method for generating loss of function mutants, especially for targeting genes belonging to large gene families. We have recently shown that RNAi functions in the moss Physcomitrella patens. We obtained stable lines that show constitutive silencing of a nuclearly localized GFP:GUS fusion protein (NLS:GFP:GUS). However, lines that display silencing of the protein do not necessarily have reduced transcript levels. Therefore, a system has been developed that silences the NLS:GFP… CONTINUE READING
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