An RNA polymerase II complex containing capping enzymes and viral oncoproteins.

@article{Cabrejos2000AnRP,
  title={An RNA polymerase II complex containing capping enzymes and viral oncoproteins.},
  author={Mar{\'i}a Eugenia Cabrejos and Edio Maldonado},
  journal={IUBMB life},
  year={2000},
  volume={50 2},
  pages={
          125-9
        }
}
The aim of this work was to identify proteins from Adenovirus 2-infected HeLa cell extracts that interact with the carboxyl-terminal domain of the largest subunit of RNA polymerase II. First, a mammalian RNA polymerase II complex was isolated from Adenovirus 2-infected HeLa cell extracts by affinity chromatography against the carboxyl-terminal domain of the largest subunit of RNA polymerase II, followed by chromatography on a Mono S fast protein liquid chromatographic column. Second, the… CONTINUE READING