An RNA interference knock-down of nitrate reductase enhances lipid biosynthesis in the diatom Phaeodactylum tricornutum.

@article{Levitan2015AnRI,
  title={An RNA interference knock-down of nitrate reductase enhances lipid biosynthesis in the diatom Phaeodactylum tricornutum.},
  author={Orly Levitan and Jorge Dinamarca and Ehud Zelzion and Maxim Y. Gorbunov and Paul G. Falkowski},
  journal={The Plant journal : for cell and molecular biology},
  year={2015},
  volume={84 5},
  pages={
          963-73
        }
}
When diatoms are stressed for inorganic nitrogen they remodel their intermediate metabolism and redirect carbon towards lipid biosynthesis. However, this response comes at a significant cost reflected in decreased photosynthetic energy conversion efficiency and growth. Here we explore a molecular genetics approach to restrict the assimilation of inorganic nitrogen by knocking down nitrate reductase (NR). The transformant strain, NR21, exhibited about 50% lower expression and activity of the… CONTINUE READING
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