An RNA extraction protocol for shellfish-borne viruses.

  title={An RNA extraction protocol for shellfish-borne viruses.},
  author={David H. Kingsley},
  journal={Journal of virological methods},
  volume={141 1},
The GPTT virus RNA extraction method, originally developed for extraction of human norovirus and hepatitis A virus RNAs from contaminated shellfish, was evaluated for extraction of RNA from Aichi virus strain A846/88 (AiV), coxsackievirus strains A9 (CAV9) and B5 (CBV5), murine norovirus (strain MNV-1), and the norovirus surrogate, feline calicivirus (FCV) strain KCD, for the purpose of RT-PCR detection within seeded oyster (Crassostrea virginica) extracts. The RT-PCR equivalent sensitivities… CONTINUE READING
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