An Order of Magnitude Faster AIP1-Associated Actin Disruption than Nucleation by the Arp2/3 Complex in Lamellipodia

@article{Tsuji2009AnOO,
  title={An Order of Magnitude Faster AIP1-Associated Actin Disruption than Nucleation by the Arp2/3 Complex in Lamellipodia},
  author={Takahiro Tsuji and Takushi Miyoshi and Chiharu Higashida and Shuh Narumiya and Naoki Watanabe},
  journal={PLoS ONE},
  year={2009},
  volume={4},
  pages={1505 - 1516}
}
The mechanism of lamellipod actin turnover is still under debate. To clarify the intracellular behavior of the recently-identified actin disruption mechanism, we examined kinetics of AIP1 using fluorescent single-molecule speckle microscopy. AIP1 is thought to cap cofilin-generated actin barbed ends. Here we demonstrate a reduction in actin-associated AIP1 in lamellipodia of cells overexpressing LIM-kinase. Moreover, actin-associated AIP1 was rapidly abolished by jasplakinolide, which… CONTINUE READING

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