An Online Four-Dimensional HIC×SEC-IM×MS Methodology for Proof-of-Concept Characterization of Antibody Drug Conjugates.

@article{Ehkirch2018AnOF,
  title={An Online Four-Dimensional HIC×SEC-IM×MS Methodology for Proof-of-Concept Characterization of Antibody Drug Conjugates.},
  author={Anthony Ehkirch and Valentina D'Atri and Florent Rouvi{\`e}re and Oscar Hernandez-Alba and Alexandre Goyon and Olivier Colas and Morgan Sarrut and Alain Beck and Davy Guillarme and Sabine Heinisch and Sarah Cianf{\'e}rani},
  journal={Analytical chemistry},
  year={2018},
  volume={90 3},
  pages={1578-1586}
}
There are currently two main techniques allowing the analytical characterization of interchain cysteine-linked antibody drug conjugates (ADCs) under native conditions, namely, hydrophobic interaction chromatography (HIC) and native mass spectrometry (MS). HIC is a chromatographic technique allowing the evaluation of drug load profile and calculation of… CONTINUE READING