An Oligonucleotide Primer System for Amplification of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Genes of Bacteria of Various Taxonomic Groups

@article{Spiridonova2004AnOP,
  title={An Oligonucleotide Primer System for Amplification of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Genes of Bacteria of Various Taxonomic Groups},
  author={Elizaveta M Spiridonova and Ivan A Berg and Tatjana V Kolganova and Ruslan N. Ivanovsky and Boris B. Kuznetsov and Tatjana P. Tourova},
  journal={Microbiology},
  year={2004},
  volume={73},
  pages={316-325}
}
Based on the analysis of GenBank nucleotide sequences of the cbbL and cbbM genes, coding for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC), the key enzyme of the Calvin cycle, a primer system was designed that allows fragments of these genes about 800 bp long to be PCR-amplified for various photo- and chemotrophic bacteria. The efficiency of the designed primer system in detection of RuBPC genes was demonstrated in PCR with DNA of taxonomically diverse bacteria… CONTINUE READING

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