An N-ethyl-N-nitrosourea screen for genes involved in variegation in the mouse.

@article{Blewitt2005AnNS,
  title={An N-ethyl-N-nitrosourea screen for genes involved in variegation in the mouse.},
  author={Marnie E. Blewitt and Nicola K Vickaryous and S Hemley and A. Marice Ashe and Timothy James Bruxner and Jost I. Preis and Ruth M Arkell and Emma Whitelaw},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 21},
  pages={7629-34}
}
We have developed a sensitized screen to identify genes involved in gene silencing, using random N-ethyl-N-nitrosourea mutagenesis on mice carrying a variegating GFP transgene. The dominant screen has produced six mutant lines, including both suppressors and enhancers of variegation. All are semidominant and five of the six are homozygous embryonic lethal. In one case, the homozygous lethality depends on sex: homozygous females die at midgestation and display abnormal DNA methylation of the X… CONTINUE READING

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