An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes

@article{Chen2017AnNR,
  title={An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes},
  author={Shun-Jia Chen and Xia Wu and Brandon Christopher Wadas and Jang-Hyun Oh and Alexander Varshavsky},
  journal={Science},
  year={2017},
  volume={355}
}
Cells synthesize glucose if deprived of it, and destroy gluconeogenic enzymes upon return to glucose-replete conditions. We found that the Gid4 subunit of the ubiquitin ligase GID in the yeast Saccharomyces cerevisiae targeted the gluconeogenic enzymes Fbp1, Icl1, and Mdh2 for degradation. Gid4 recognized the N-terminal proline (Pro) residue and the ~5-residue-long adjacent sequence motifs. Pck1, the fourth gluconeogenic enzyme, contains Pro at position 2; Gid4 directly or indirectly recognized… CONTINUE READING

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