An MDR-EGFP gene fusion allows for direct cellular localization, function and stability assessment of P-glycoprotein.

@article{Ptriz2004AnMG,
  title={An MDR-EGFP gene fusion allows for direct cellular localization, function and stability assessment of P-glycoprotein.},
  author={Jordi P{\'e}triz and Michael M. Gottesman and Josep M Aran},
  journal={Current drug delivery},
  year={2004},
  volume={1 1},
  pages={43-56}
}
In cancer and AIDS, overexpression of the MDR1 gene has important implications in the design of chemotherapy protocols because of the ability of its product, the ATP-dependent drug efflux pump P-glycoprotein (Pgp), to confer selective advantage to tumor and HIV-infected cells in the form of multidrug resistance. To study Pgp expression and physiology, we designed a translational fusion between the MDR1 and enhanced green fluorescent protein (EGFP) genes. The chimeric protein, Pgp-EGFP, was… CONTINUE READING