An Investigation of the Midgut K+ Pump of the Tobacco Hornworm (manduca Sexta) Using Specific Inhibitors and Amphotericin B

@article{Schirmanns1994AnIO,
  title={An Investigation of the Midgut K+ Pump of the Tobacco Hornworm (manduca Sexta) Using Specific Inhibitors and Amphotericin B},
  author={Schirmanns and Zeiske},
  journal={The Journal of experimental biology},
  year={1994},
  volume={188 1},
  pages={191-204}
}
Active K+ secretion in isolated posterior midguts of Manduca sexta was studied by measuring the short-circuit current. One aim of this study was to verify the postulate from biochemical reports that the cooperative apical arrangement of a vacuolar-type H+-ATPase (V-ATPase) and a K+/H+ antiporter drive the short-circuit current. Hence, we tested several specific inhibitors of the V-ATPase on the in vitro midgut preparation. Nitrate, bafilomycin A1, 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl… CONTINUE READING