An Integrated Model of the Transcription Complex in Elongation, Termination, and Editing

@article{Hippel1998AnIM,
  title={An Integrated Model of the Transcription Complex in Elongation, Termination, and Editing},
  author={Peter H. von Hippel},
  journal={Science},
  year={1998},
  volume={281},
  pages={660-665}
}
Recent findings now allow the development of an integrated model of the thermodynamic, kinetic, and structural properties of the transcription complex in the elongation, termination, and editing phases of transcript formation. This model provides an operational framework for placing known facts and can be extended and modified to incorporate new advances. The most complete information about transcriptional mechanisms and their control continues to come from the Escherichia coli system, upon… 
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The regulatory molecules that are known to influence the elongation/termination decision by RNA polymerase (RNAP) are described, with the emphasis on the most recent findings and on the mechanism of ‘‘active’’ regulators whose actions are not limited to changes in RNA folding.
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  • 1999
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A structure-function model of the ternary elongation complex ofRNAP is suggested, if not at a defined structural level, then at least as a conceptual view, such that key components of RNAP are defined operationally on the basis of compelling biochemical, protein chemical, and genetic data.
Transcription termination and anti‐termination in E. coli
TLDR
Basic principles are reviewed and attempt to unify into basic principles the remarkable recent progress in understanding transcription termination and anti‐termination in Escherichia coli.
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TLDR
It is shown that the formation of a stable elongation complex at any particular template position can be treated as an equilibrium process, and that semi-quantitative dissociation constants can be estimated for the complex by using a gel band-shift assay to monitor the binding of the RNA oligomer to the complex.
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