An Informatics-assisted Label-free Approach for Personalized Tissue Membrane Proteomics: Case Study on Colorectal Cancer*

@inproceedings{Han2011AnIL,
  title={An Informatics-assisted Label-free Approach for Personalized Tissue Membrane Proteomics: Case Study on Colorectal Cancer*},
  author={Chia-Li Han and Jinn-Shiun Chen and Err-Cheng Chan and Chien-Peng Wu and Kun-Hsing Yu and K T Chen and Chih-Chiang Tsou and Chia-Feng Tsai and Chih-Wei Chien and Yung-Bin Kuo and P. Lin and Jau-Song Yu and Chuen Hsueh and Meili Chen and Chung-Chuan Chan and Yu-sun Chang and Yu-Ju Chen},
  booktitle={Molecular & cellular proteomics : MCP},
  year={2011}
}
We developed a multiplexed label-free quantification strategy, which integrates an efficient gel-assisted digestion protocol, high-performance liquid chromatography tandem MS analysis, and a bioinformatics alignment method to determine personalized proteomic profiles for membrane proteins in human tissues. This strategy provided accurate (6% error) and reproducible (34% relative S.D.) quantification of three independently purified membrane fractions from the same human colorectal cancer (CRC… CONTINUE READING
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