An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.

@article{Li2016AnIM,
  title={An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii.},
  author={Xiaobo Li and Ru Zhang and Weronika Patena and Spencer S Gang and Sean R. Blum and Nina Ivanova and Rebecca Yue and Jacob M Robertson and Paul A Lefebvre and Sorel T Fitz-Gibbon and Arthur R Grossman and Martin C Jonikas},
  journal={The Plant cell},
  year={2016},
  volume={28 2},
  pages={367-87}
}
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting biological processes in photosynthetic eukaryotes. However, its usefulness has been limited by difficulties in obtaining mutants in specific genes of interest. To allow generation of large numbers of mapped mutants, we developed high-throughput methods that (1) enable easy maintenance of tens of thousands of Chlamydomonas strains by propagation on agar media and by cryogenic storage, (2) identify mutagenic… CONTINUE READING
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