An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

@inproceedings{Geng2009AnIM,
  title={An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum},
  author={Shi-Zhong Geng and Xin-an Jiao and Zhiming Pan and Xiao-juan Chen and Xiao-Ming Zhang and Xiang Chen},
  booktitle={Journal of biomedicine & biotechnology},
  year={2009}
}
An asd-deleted (Deltaasd) mutant of Salmonella enterica serovar Pullorum (SP) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the Red Disruption system. The asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the CmR gene. Based on the balanced lethal host-vector system, the phenotype of the Deltaasd mutant was further defined. The improved method was simpler and more effective than… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 26 references

Similar Papers

Loading similar papers…