An Improved High-Throughput Lipid Extraction Method for the Analysis of Human Brain Lipids

@article{Abbott2013AnIH,
  title={An Improved High-Throughput Lipid Extraction Method for the Analysis of Human Brain Lipids},
  author={Sarah K. Abbott and Andrew M. Jenner and Todd W Mitchell and Simon H J Brown and Glenda M Halliday and Brett Garner},
  journal={Lipids},
  year={2013},
  volume={48},
  pages={307-318}
}
We have developed a protocol suitable for high-throughput lipidomic analysis of human brain samples. The traditional Folch extraction (using chloroform and glass–glass homogenization) was compared to a high-throughput method combining methyl-tert-butyl ether (MTBE) extraction with mechanical homogenization utilizing ceramic beads. This high-throughput method significantly reduced sample handling time and increased efficiency compared to glass–glass homogenizing. Furthermore, replacing… CONTINUE READING
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