An Improved β-Lactamase Reporter Assay: Multiplexing with a Cytotoxicity Readout for Enhanced Accuracy of Hit Identification

@article{Hallis2007AnI,
  title={An Improved β-Lactamase Reporter Assay: Multiplexing with a Cytotoxicity Readout for Enhanced Accuracy of Hit Identification},
  author={Tina M. Hallis and Andrew L Kopp and Jasmin R Gibson and Connie S. Lebakken and Michael L. Hancock and Kaitlyn Vandenheuvel-Kramer and Tammy C. Turek-Etienne},
  journal={Journal of Biomolecular Screening},
  year={2007},
  volume={12},
  pages={635 - 644}
}
A problem inherent to the use of cellular assays for drug discovery is their sensitivity to cytotoxic compounds, which can result in false hits from certain compound screens. To alleviate the need to follow-up hits from a reporter assay with a separate cytotoxicity assay, the authors have developed a multiplexed assay that combines the readout of a β-lactamase reporter with that of a homogeneous cytotoxicity indicator. Important aspects to the development of the multiplexed format are addressed… CONTINUE READING

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