An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid.

@article{Linxiang2004AnHA,
  title={An HPLC assay of hydroxyl radicals by the hydroxylation reaction of terephthalic acid.},
  author={Li Linxiang and Yoshihiro Abe and Yukiko Nagasawa and Risa Kudo and Noriko Usui and Kazuhiro Imai and Tadahiko Mashino and Masataka Mochizuki and Naoki Miyata},
  journal={Biomedical chromatography : BMC},
  year={2004},
  volume={18 7},
  pages={470-4}
}
An HPLC assay for hydroxyl radicals is described. The hydroxyl radical was trapped by terephthalic acid (non-fluorescent), and 2-hydroxyl terephthalic acid (fluorescent) was quantitated by HPLC-fluorescence detection. At a terephthalic acid concentration of 4.25 mmol/L, the hydroxyl radical formed in the Fenton reaction was successfully assayed in the concentration range of hydrogen peroxide of 2.5-50 micro mol/L, where the concentration of Fe(II) was 50 micro mol/L. The fluorescence of 2… CONTINUE READING
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