An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.

@article{Lyell2017AnET,
  title={An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.},
  author={Noreen L. Lyell and Alecia N. Septer and Anne K. Dunn and Drew Duckett and Julie L Stoudenmire and Eric V. Stabb},
  journal={Applied and environmental microbiology},
  year={2017},
  volume={83 5}
}
Libraries of defined mutants are valuable research tools but necessarily lack gene knockouts that are lethal under the conditions used in library construction. In this study, we augmented a Vibrio fischeri mutant library generated on a rich medium (LBS, which contains [per liter] 10 g of tryptone, 5 g of yeast extract, 20 g of NaCl, and 50 mM Tris [pH 7.5… CONTINUE READING