An Escherichia coli mutant defective in lipid export.

@article{Doerrler2001AnEC,
  title={An Escherichia coli mutant defective in lipid export.},
  author={William T. Doerrler and Mary C. Reedy and Christian R. H. Raetz},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 15},
  pages={
          11461-4
        }
}
Escherichia coli phospholipids and lipopolysaccharide, made on the inner surface of the inner membrane, are rapidly transported to the outer membrane by mechanisms that are not well characterized. We now report a temperature-sensitive mutant (WD2) with an A270T substitution in a trans-membrane region of the ABC transporter MsbA. As shown by (32)P(i) and (14)C-acetate labeling, export of all major lipids to the outer membrane is inhibited by approximately 90% in WD2 after 30 min at 44 degrees C… CONTINUE READING

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