An Efficient Rice Transformation System Utilizing Mature Seed-derived Explants and a Portable, Inexpensive Particle Bombardment Device

@article{Sudhakar2004AnER,
  title={An Efficient Rice Transformation System Utilizing Mature Seed-derived Explants and a Portable, Inexpensive Particle Bombardment Device},
  author={Durailagaraja Sudhakar and Le Tan Duc and Bui Ba Bong and Porntip Tinjuangjun and Shahina Bano Maqbool and M. N250241ez Valdez and Richard A. Jefferson and Paul Christou},
  journal={Transgenic Research},
  year={2004},
  volume={7},
  pages={289-294}
}
We developed a practical and efficient gene transfer system for indica rice utilizing mature-seed derived explants and a simple bombardment device which uses compressed helium for accelerating DNA-coated metal particles. Unlike instruments which have been described in the literature previously, this new bombardment device, which is an improvement of the particle inflow concept, does not require vacuum. This attribute simplifies the transformation procedure significantly and it makes rice… CONTINUE READING

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