An Efficient Procedure for Regeneration from Leaf-derived Calluses of Lonicera macranthoides ‘ Jincuilei ’ , an Important Medicinal Plant

@inproceedings{Wang2009AnEP,
  title={An Efficient Procedure for Regeneration from Leaf-derived Calluses of Lonicera macranthoides ‘ Jincuilei ’ , an Important Medicinal Plant},
  author={Xiaoming Wang and Jianjun Chen},
  year={2009}
}
‘Jincuilei’ is a mutant selected from Lonicera macranthoides Hand.-Mazz. It produces abundant flowers that never open with a chlorogenic acid (CGA) content up to 6.0%. Propagation through rooting or grafting has only a 30% survival rate. This study was undertaken to establish an efficient protocol for rapidly regenerating this mutant. Leaf explants were inoculated on Gamborg’s B5 medium supplemented with different concentrations of 6-benzyladenine (BA) and 2,4-dichlorophenozyacetic acid (2,4-D… CONTINUE READING
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The optimal combination for callus induction was 4.4 mM BA with 2.26 mM 2,4-D , which resulted in 86.7% of leaf explants producing calluses in 4 weeks .
The optimal combination for callus induction was 4.4 mM BA with 2.26 mM 2,4-D , which resulted in 86.7% of leaf explants producing calluses in 4 weeks .
Flos lonicerae contains a series of water - soluble phenolic compounds with 3-O - ceffeoyl - D - quinic acid or chlorogenic acid ( CGA ) being a major ingredient .
Flos lonicerae contains a series of water - soluble phenolic compounds with 3-O - ceffeoyl - D - quinic acid or chlorogenic acid ( CGA ) being a major ingredient .
The optimal combination for callus induction was 4.4 mM BA with 2.26 mM 2,4-D , which resulted in 86.7% of leaf explants producing calluses in 4 weeks .
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