An Efficient Low Cost Method for Gene Transfer to T Lymphocytes

@inproceedings{Chicaybam2013AnEL,
  title={An Efficient Low Cost Method for Gene Transfer to T Lymphocytes},
  author={Leonardo Chicaybam and Andressa Laino Sodr{\'e} and Bianca Azevedo Curzio and Martin H Bonamino},
  booktitle={PloS one},
  year={2013}
}
UNLABELLED Gene transfer to T lymphocytes has historically relied on retro and lentivirus, but recently transposon-based gene transfer is rising as a simpler and straight forward approach to achieve stable transgene expression. Transfer of expression cassettes to T lymphocytes remains challenging, being based mainly on commercial kits. AIMS We herein report a convenient and affordable method based on in house made buffers, generic cuvettes and utilization of the widely available Lonza… CONTINUE READING

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