An Efficient In Vitro Assay for Acetylcholinesterase Reactivators Using Immobilized Enzyme

@article{Trammel1984AnEI,
  title={An Efficient In Vitro Assay for Acetylcholinesterase Reactivators Using Immobilized Enzyme},
  author={Andy M. Trammel and John E. Simmons and Ronald T. Borchardt},
  journal={Pharmaceutical Research},
  year={1984},
  volume={1},
  pages={115-120}
}
A new and efficient in vitro assay for evaluating reactivators of organophosphate-inhibited acetylcholinesterase has been developed. Low density polyethylene beads (4 mm) were func-tionalized to terminal aldehydes and used to immobilize acetylcholinesterase (AChE, Electrophorus electricus, E.G. 3.1.1.7) via a stable Schiff base link. AChE activity in… CONTINUE READING