An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans

@inproceedings{Nguyen2017AnER,
  title={An Efficient, Rapid, and Recyclable System for CRISPR-Mediated Genome Editing in Candida albicans},
  author={Namkha Nguyen and Morgan M. F. Quail and Aaron D. Hernday},
  booktitle={mSphere},
  year={2017}
}
Candida albicans is the most common fungal pathogen of humans. Historically, molecular genetic analysis of this important pathogen has been hampered by the lack of stable plasmids or meiotic cell division, limited selectable markers, and inefficient methods for generating gene knockouts. The recent development of clustered regularly interspaced short palindromic repeat(s) (CRISPR)-based tools for use with C. albicans has opened the door to more efficient genome editing; however, previously… CONTINUE READING