An ELISA based on whole virus for the detection of antibodies to small-ruminant lentiviruses.

@article{Zanoni1994AnEB,
  title={An ELISA based on whole virus for the detection of antibodies to small-ruminant lentiviruses.},
  author={Reto Zanoni and H. R. Vogt and Birte Pohl and Joan Boettcher and W Bommeli and Ernst Peterhans},
  journal={Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B},
  year={1994},
  volume={41 10},
  pages={662-9}
}
A new ELISA kit was developed, based on highly purified whole-virus antigen derived from the Swiss maedi-visna virus strain OLV. The sensitivity, specificity and accuracy of this assay were compared with that of an established ELISA based on recombinant GAG (group-specific antigens)-GST (glutathione S-transferase) fusion protein expressed in E. coli (GAG-GST ELISA). The whole-virus ELISA exhibits at least comparable specificity (99.3%) but higher sensitivity (98.6 versus 86.3%) and agreement… CONTINUE READING
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