An Assessment of Endocrine Disrupting Activity Changes during Wastewater Treatment through the Use of Bioassays and Chemical Measurements

  title={An Assessment of Endocrine Disrupting Activity Changes during Wastewater Treatment through the Use of Bioassays and Chemical Measurements},
  author={J{\"o}rg E. Drewes and Jocelyn D. C. Hemming and Sarah J. Ladenburger and James J. Schauer and William C. Sonzogni},
  journal={Water Environment Research},
The objective of this study was to assess the removal efficiencies of secondary wastewater treatment processes for compounds causing endocrine disrupting activity. The study used bioassays and chemical measurements, such as gas chromatography with mass spectrometry and enzyme immunosorbent assays. A total of seven full‐scale water reclamation facilities using different unit operations and two pilot‐scale membrane bioreactors were examined. Findings of this study imply that estrogenic disrupting… 
Assessment of trace organic chemical removal by a membrane bioreactor using gas chromatography/mass spectrometry and a yeast screen bioassay
It was concluded that the main removal pathway was biodegradation, but sorption to biomass may also be important, particularly for triclosan and 4‐tert‐octylphenol.
The Impact of Endocrine Disrupting Chemicals on the Environmental and their Potential Biotransformation by White-rot Fungi and their Oxidative Enzymes
The search of new technologies suitable for the treatment of wastewater containing endocrine disrupting chemicals (EDCs) such as bisphenol A (BPA), triclosan (TCS), estrone (E1), 17β-estradiol (E2)
Comparison of the removal efficiency of endocrine disrupting compounds between two lab-scale treatment processes
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  • Biology
    2011 International Conference on Remote Sensing, Environment and Transportation Engineering
  • 2011
The EDCs removal efficiency of AC, especially the granular form, might more effective than BCO process, and the antiandrogenic activities showed no significant difference between before and after BCO treatment.
Application of radio-immunoassays to assess the fate of estrogen EDCs in full scale wastewater treatment plants
  • S. Surujlal-Naicker, F. Bux
  • Biology
    Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering
  • 2013
Radio-immunoassays (RIA) were assessed to determine the fate of estradiol in a laboratory batch test and the three natural estrogens in wastewater treatment plants (WWTPs) with different types of configurations, indicating that the RIA can be employed as a rapid technique for detection of natural estrogen in water.
Tools to detect estrogenic activity in environmental waters
Some bioassay techniques are now sufficiently advanced that they can be used either as a cost-effective first-pass detection system or in combination with standard analytical methods to measure estrogenic pollutants in environmental waters.
In Vitro and Immunological Assessment of the Estrogenic Activity and Concentrations of 17β-Estradiol, Estrone, and Ethinyl Estradiol in Treated Effluent from 45 Wastewater Treatment Plants in Victoria, Australia
The levels of estrogenic activity observed were comparable with the range recently reported in Australia and New Zealand using human estrogen receptor-based assays (“not detected” to ~10 ng/l EEQ) and the low/no bioassay response was confirmed by the chemical assessment of estradiol, estrone, and ethinylEstradiol concentrations by ELISA, which returned concentrations for the most part below 10 NG/l.
Toxicity identification fractionation of environmental estrogens in waste water and sludge using gas and liquid chromatography coupled to mass spectrometry and recombinant yeast assay
The integrated procedure herein proposed can be used as a screening method to evaluate estrogenic compounds in STPs and to survey faecal elimination.


Removal mechanisms of endocrine disrupting compounds (steroids) during soil aquifer treatment.
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  • Engineering
    Water science and technology : a journal of the International Association on Water Pollution Research
  • 2004
The objective of this study was to determine the primary removal mechanisms of endocrine disruptors such as steroidal hormones present in reclaimed water, specifically 17beta-estradiol, estriol, and
Removal of endocrine-disrupting chemicals in activated sludge treatment works.
While water purification techniques such as UV or activated charcoal could significantly remove these microorganic contaminants, the high costs involved suggest that research into the potential for treatment optimization should receive more attention.
Identification of Estrogenic Chemicals in STW Effluent. 1. Chemical Fractionation and in Vitro Biological Screening
A fractionation system, combined with an in vitro assay for detecting estrogenic activity, was developed in order to isolate and identify the major estrogenic chemicals present in seven
Estrogen receptor agonist fate during wastewater and biosolids treatment processes: a mass balance analysis.
A correlation was observed between the estrogenicity of mixed liquor suspended solids and aerobic sludge age and suggests that wastewater treatment facilities can be designed and operated to enhance the sorption and removal of estrogenic compounds from the liquid phase.
Fate of estrogens in a municipal sewage treatment plant.
The main outcome of the study was that a common municipal STP with an activated sludge system for nitrification and denitrification including sludge recirculation can appreciably eliminate natural and synthetic estrogens.
Identification and quantification of estrogen receptor agonists in wastewater effluents.
Both bioassay-directed fractionation results and comparison of ER agonist concentrations, adjusted for their known relative potencies, support the conclusion that E2 and EE2 were the dominant environmental estrogens in water samples from mid-Michigan and Lake Mead, NV.
Monitoring Natural and Synthetic Estrogens at Activated Sludge Sewage Treatment Plants and in a Receiving River Water
Sewage treatment plant (STP) effluents with primarily domestic inputs are strongly suspected to be an important source of natural and synthetic estrogens contaminating the aquatic environment. Even a
Analysis and occurrence of estrogenic hormones and their glucuronides in surface water and waste water in The Netherlands.
Analytical Methods for Detection of Selected Estrogenic Compounds in Aqueous Mixtures
Both natural estrogens and synthetic compounds that mimic estrogen can reach the aquatic environment through wastewater discharges. Because nonylphenol (NP), octylphenol (OP), nonylphenol
Analysis of estrogenic hormones in municipal wastewater effluent and surface water using enzyme‐linked immunosorbent assay and gas chromatography/tandem mass spectrometry
Results from ELISA analysis of estrogenic hormones in secondary wastewater effluent indicate concentrations comparable to those that cause vitellogenesis in fish, which provides a relatively simple and practical method of assessing the fate of estrogenIC hormones in engineered and natural systems.