An Animal Able To Tolerate D2O

  title={An Animal Able To Tolerate D2O},
  author={Gabriel Jos{\'e} Carli and Danyel Fernandes Contiliani and Silvana Giuliatti and Tiago Campos Pereira},
It is possible to gain a deeper insight into the role of water in biology by using physicochemical variant molecules, such as deuterium oxide (D2O); however, D2O is toxic to multicellular organisms in high concentrations. By using a unique desiccation‐rehydration process, we demonstrate that the anhydrobiotic nematode Panagrolaimus superbus is able to tolerate and proliferate in 99 % D2O. Moreover, we analysed P. superbus’ water‐channel protein (aquaporin; AQP), which is associated with… 


What Are Aquaporins For?
It is concluded that in a range of situations at the cellular, subcellular and tissue levels the SPH cannot satisfactorily account for the presence of AQPs and suggested that this sensor hypothesis can provide an explanation of many basic processes in which AQPs are already implicated.
Mutations in a single signaling pathway allow cell growth in heavy water.
Although the heat-shock response system upregulates various chaperones and other stress-relieving enzymes, it is found that activation of this pathway does not offer any fitness advantage to the cells under the solvent replaced conditions, however, limiting the D2O-triggered activation of the cell integrity pathway allows cell growth when H2O is completely replaced with D1O.
The evolutionary aspects of aquaporin family.
This review focuses on the evolutionary aspects of AQP from bacteria to humans in view of the structural and functional diversities of AQPs.
Resurrecting Van Leeuwenhoek's rotifers: a reappraisal of the role of disaccharides in anhydrobiosis.
  • A. Tunnacliffe, J. Lapinski
  • Biology, Medicine
    Philosophical transactions of the Royal Society of London. Series B, Biological sciences
  • 2003
The purpose of this review is to examine what has been learned since then about the extreme desiccation tolerance in rotifers and how this compares with the understanding of anhydrobiosis in other organisms.
Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae
Yeast is a versatile system for functional studies of aquaporins, and it can be developed to screen for compounds of potential pharmacological use, suggesting that yeast can potentially be used for screening of putative aquaporin inhibitors.
Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
Functional screening by RNA interference for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus suggests that multiple genes contribute to anhydRobiosis in P. superbus.
Molecular Cloning and Characterization of AqpZ, a Water Channel from Escherichia coli(*)
Phylogenetic comparison of aquaporins and homologs revealed a large separation between aqpZ and glpF, consistent with an ancient gene divergence.
Deuterium and its impact on living organisms
Although the effects of deuterium on living organisms are, in general, negative, some of its applications are of great biotechnological potential, as is the case of stable isotope-labelled compounds or deuterated drugs.
Pharmacological uses and perspectives of heavy water and deuterated compounds.
D2O and deuterated drugs are widely used in studies of metabolism of drugs and toxic substances in humans and other animals, and most are more resistant to metabolic changes, especially those changes mediated by cytochrome P450 systems.
Visualization of AqpZ-Mediated Water Permeability in Escherichia coli by Cryoelectron Microscopy
It is shown that the AqpZ water channel rapidly mediates large water fluxes in response to sudden changes in extracellular osmolarity, and functional expression of a prokaryotic water channel is demonstrated for the first time.