• Corpus ID: 83253538

An Analysis of Glycolytic Enzymes in the Cellular Response to Metal Toxicity

  title={An Analysis of Glycolytic Enzymes in the Cellular Response to Metal Toxicity},
  author={Anupama Shanmuganathan},
Metal toxicity is implicated in neurotoxicity, nephrotoxicity, aging and cancer. Protein oxidation resulting from oxidative stress is now known to be involved in metal toxicity. However, proteomic responses to metal induced oxidative stress have not been characterized. By using the yeast as a model, we characterized these changes occurring in response to sub-lethal doses of metals. Several proteins involved in protein synthesis, ribosome assembly decreased while antioxidant defenses, proteins… 

Figures and Tables from this paper

Analysis of cellular responses to heavy metal-induced stress in Saccharomyces cerevisiae
A cytometric methodology is developed to assess the autophagic flow, that is less labor-intensive and more dynamic than the traditional Western blot-based method, and is able to decipher the factors of cell fate decision with heavy metal-induced oxidative stress in S. cerevisiae.


Carbonylation of glycolytic proteins is a key response to drug-induced oxidative stress and apoptosis
It is demonstrated that protein carbonylation can be rapidly induced through cytotoxic drug treatment and may specifically inhibit the glycolytic pathway, which has severe implications for cell function.
Role of the glutathione/glutaredoxin and thioredoxin systems in yeast growth and response to stress conditions
  • C. Grant
  • Biology
    Molecular microbiology
  • 2001
This review describes recent findings in the lower eukaryote Saccharomyces cerevisiae that are leading to a better understanding of their role in redox homeostasis in eUKaryotic cell metabolism.
Metal toxicity in yeasts and the role of oxidative stress.
  • S. Avery
  • Biology
    Advances in applied microbiology
  • 2001
Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiae.
Protein S-thiolation appears to serve an adaptive function during exposure to an oxidative stress by reprogramming metabolism and protecting protein synthesis against irreversible oxidation, and is proposed to result in glucose equivalents entering the pentose phosphate pathway for the generation of NADPH.
Mitochondrial Hsp60, Resistance to Oxidative Stress, and the Labile Iron Pool Are Closely Connected in Saccharomyces cerevisiae *
The results indicate that the role of Hsp60 in oxidative-stress defense is explained by protection of several Fe/S proteins, which prevent the release of iron ions and thereby avert further damage.
Cadmium-inducible Expression of the Yeast GSH1 Gene Requires a Functional Sulfur-Amino Acid Regulatory Network*
Evidence is presented that the transcription factors involved in controlling the network of sulfur amino acid metabolism genes are also responsible for regulating GSH1 expression in response to cadmium.
Oxidative mechanisms in the toxicity of metal ions.