An ATP-independent strategy for amide bond formation in antibiotic biosynthesis.

@article{Funabashi2010AnAS,
  title={An ATP-independent strategy for amide bond formation in antibiotic biosynthesis.},
  author={Masanori Funabashi and Zhaoyong Yang and Koichi Nonaka and Masahiko Hosobuchi and Yoko Fujita and Tomoyuki Shibata and Xiuling Chi and Steven G Van Lanen},
  journal={Nature chemical biology},
  year={2010},
  volume={6 8},
  pages={581-6}
}
A-503083 B, a capuramycin-type antibiotic, contains an L-aminocaprolactam and an unsaturated hexuronic acid that are linked via an amide bond. A putative class C beta-lactamase (CapW) was identified within the biosynthetic gene cluster that-in contrast to the expected beta-lactamase activity-catalyzed an amide-ester exchange reaction to eliminate the L-aminocaprolactam with concomitant generation of a small but significant amount of the glyceryl ester derivative of A-503083 B, suggesting a… CONTINUE READING