An ATP-ADP switch in MuB controls progression of the Mu transposition pathway.

@article{Yamauchi1998AnAS,
  title={An ATP-ADP switch in MuB controls progression of the Mu transposition pathway.},
  author={Meire N. Yamauchi and Tania A. Baker},
  journal={The EMBO journal},
  year={1998},
  volume={17 18},
  pages={5509-18}
}
MuB protein, an ATP-dependent DNA-binding protein, collaborates with Mu transposase to promote efficient transposition. MuB binds target DNA, delivers this target DNA segment to transposase and activates transposase's catalytic functions. Using ATP-bound, ADP-bound and ATPase-defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein, important for transposition. We found that both MuB-ADP and MuB-ATP stimulate transposase, whereas only… CONTINUE READING

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An ATP - ADP switch in MuB controls progression of the Mu transposition pathway .
We found that both MuB - ADP and MuB - ATP stimulate transposase , whereas only MuB - ATP binds with high affinity to DNA .
We found that both MuB - ADP and MuB - ATP stimulate transposase , whereas only MuB - ATP binds with high affinity to DNA .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
We found that both MuB - ADP and MuB - ATP stimulate transposase , whereas only MuB - ATP binds with high affinity to DNA .
An ATP - ADP switch in MuB controls progression of the Mu transposition pathway .
We found that both MuB - ADP and MuB - ATP stimulate transposase , whereas only MuB - ATP binds with high affinity to DNA .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
Using ATP - bound , ADP - bound and ATPase - defective MuB proteins we investigated how nucleotide binding and hydrolysis control the activities of MuB protein , important for transposition .
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