Amyloid-β(1–42) Protofibrils Formed in Modified Artificial Cerebrospinal Fluid Bind and Activate Microglia

  title={Amyloid-β(1–42) Protofibrils Formed in Modified Artificial Cerebrospinal Fluid Bind and Activate Microglia},
  author={Geeta S. Paranjape and Shana E. Terrill and L. Gouwens and B. M. Ruck and M. R. Nichols},
  journal={Journal of Neuroimmune Pharmacology},
  • Geeta S. Paranjape, Shana E. Terrill, +2 authors M. R. Nichols
  • Published 2012
  • Biology, Medicine
  • Journal of Neuroimmune Pharmacology
  • Soluble aggregated forms of amyloid-β protein (Aβ) have garnered significant attention recently for their role in Alzheimer’s disease (AD. [...] Key Method A simple phosphate/bicarbonate buffer system was prepared that maintained the ionic strength and cell compatibility of F-12 medium but did not contain numerous supplements that interfere with spectroscopic analyses of Aβ protofibrils. Reconstitution of Aβ(1–42) in aCSF and isolation with size exclusion chromatography (SEC) revealed curvilinear β-sheet…Expand Abstract


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