Amyloid-β(1–42) Protofibrils Formed in Modified Artificial Cerebrospinal Fluid Bind and Activate Microglia

@article{Paranjape2012Amyloid142PF,
  title={Amyloid-β(1–42) Protofibrils Formed in Modified Artificial Cerebrospinal Fluid Bind and Activate Microglia},
  author={Geeta S. Paranjape and Shana E. Terrill and L. Gouwens and B. M. Ruck and M. R. Nichols},
  journal={Journal of Neuroimmune Pharmacology},
  year={2012},
  volume={8},
  pages={312-322}
}
  • Geeta S. Paranjape, Shana E. Terrill, +2 authors M. R. Nichols
  • Published 2012
  • Biology, Medicine
  • Journal of Neuroimmune Pharmacology
  • Soluble aggregated forms of amyloid-β protein (Aβ) have garnered significant attention recently for their role in Alzheimer’s disease (AD. [...] Key Method A simple phosphate/bicarbonate buffer system was prepared that maintained the ionic strength and cell compatibility of F-12 medium but did not contain numerous supplements that interfere with spectroscopic analyses of Aβ protofibrils. Reconstitution of Aβ(1–42) in aCSF and isolation with size exclusion chromatography (SEC) revealed curvilinear β-sheet…Expand Abstract

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 41 REFERENCES
    Isolated amyloid-β(1-42) protofibrils, but not isolated fibrils, are robust stimulators of microglia.
    • 50
    Amyloid β-Protein Dimers Rapidly Form Stable Synaptotoxic Protofibrils
    • 217
    • Highly Influential
    • PDF
    Assembly of Aβ Amyloid Protofibrils: An in Vitro Model for a Possible Early Event in Alzheimer's Disease†
    • 419
    • Highly Influential
    • PDF
    Amyloid β-Protein Fibrillogenesis
    • 135
    • Highly Influential
    • PDF
    Protofibrils of amyloid β-protein inhibit specific K+ currents in neocortical cultures
    • 69
    • Highly Influential
    Preparation and characterization of toxic Aβ aggregates for structural and functional studies in Alzheimer's disease research
    • 162
    • PDF