Amylase mRNA expression in Crassostrea gigas during feeding cycles

@article{Moal2000AmylaseME,
  title={Amylase mRNA expression in Crassostrea gigas during feeding cycles},
  author={J Moal and J. Y. Daniel and Daniel Y. Sellos and Alain Van Wormhoudt and Jean François Samain},
  journal={Journal of Comparative Physiology B},
  year={2000},
  volume={170},
  pages={21-26}
}
Abstract A Crassostrea gigas digestive gland copy DNA (cDNA) library constructed in the λ phage ZapII (Stratagene, La Jola, USA) was screened with an amylase heterologous probe. To get access to the complete cDNA, a polymerase chain reaction extension was conducted using DNA extracted from the phages. The complete cDNA sequence is 1688 base pairs (EMBL = Y08370). The deduced protein sequence is 519 aminoacids long with a 19 aminoacid signal peptide. Similarity with Pectenmaximus amylase is 72… CONTINUE READING

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