Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction.

@article{Dong2012AmplifiedDO,
  title={Amplified detection of nucleic acid by G-quadruplex based hybridization chain reaction.},
  author={Juan E Dong and Xin Cui and Yun Deng and Zhuo Tang},
  journal={Biosensors & bioelectronics},
  year={2012},
  volume={38 1},
  pages={258-63}
}
A protein-free, isothermal, self-amplified nucleic acid sensing system which was a G-quadruplex integrated hybridization chain reaction (GQ-HCR) system was developed. The G-quadruplex was closed two-thirds in the loop and one-third in the stem of one of the GQ-HCR hairpin probes. In the absence of the target molecule, the GQ-HCR probes stayed as inactive meta-stable hairpin structures and the G-quadruplex was inert. Reversely, the GQ-HCR probes could be cross-opened to start a hybridization… CONTINUE READING