Amplified Silencing

  title={Amplified Silencing},
  author={David C. Baulcombe},
  pages={199 - 200}
  • D. Baulcombe
  • Published 12 January 2007
  • Biology, Medicine
  • Science
Silencing Seed Dormancy Genes to Mitigate Risk of Transgene Flow to Weedy Rice
It is revealed that the author's father and stepmother had a falling-out over the use of foul-mouthed language that resulted in the publication of disparaging statements about each other. Expand
MicroRNAs in Development
  • T. Dalmay
  • Biology
  • Methods in Molecular Biology
  • 2011
Digoxygenin-labeled locked nucleic acid oligos have been used to bind strongly and specifically to miR targets and make the identification of the precise spatiotemporal expression of these molecules possible. Expand
Toward a quarter century of pathogen-derived resistance and practical approaches to plant virus disease control.
Applying the concept of PDR provides unique opportunities for developing virus-resistant crops and implementing efficient and environmentally sound management approaches to mitigate the impact of virus diseases. Expand
RNA interference, transcriptional gene silencing, virus induced gene silencing, and micro RNAs comprise a series of mechanisms capable of suppressing gene expression in plants. These mechanismsExpand
RNA silencing can explain chlorotic infection patterns on plant leaves
This work shows that RNA silencing and virus growth within a cell, and the local spread of virions and siRNAs between cells can explain the heterogeneous spread of virus in leaf tissue, and therewith the observed infection patterns in plants. Expand
Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants
An in vitro system that recapitulates the production of secondary siRNAs that are physiologically important in plants and shows that a combination of four plant factors promotes physical recruitment of RDR6 to the target RNA, and elucidate the molecular events necessary for secondary siRNA biogenesis in plants. Expand
Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification in Arabidopsis
An in vitro siRNA amplification system is established that RNA-dependent RNA polymerase proteins are specifically recruited to targets in coordination with two plant-specific proteins, and it is demonstrated that RDR6 is recruited to a cleavage fragment by 22-nt siRNA–containing AGO1-RISCs in coordinationWith SGS3 and SDE5. Expand
Transcriptome analysis of differentially expressed genes in chrysanthemum MET1 RNA interference lines.
  • Dong-Ru Kang, Yi Zhu, +5 authors Zi-Cheng Wang
  • Medicine
  • Physiology and molecular biology of plants : an international journal of functional plant biology
  • 2021
This study reveals the mechanism of CmMET1 interference on the growth and development of chrysanthemum at the transcriptional level, which provides the basis for further research on the epigenetic regulation mechanism in flower induction and development. Expand


Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans
Analysis of endogenous small RNAs indicated that a fraction derive from a biosynthetic mechanism that is similar to that of secondary siRNAs formed during RNAi, suggesting that small antisense transcripts derived from cellular messenger RNAs by RdRP activity may have key roles in cellular regulation. Expand
Secondary siRNAs Result from Unprimed RNA Synthesis and Form a Distinct Class
Cloned secondary siRNAs from transgenic C. elegans lines expressing a single 22-nucleotide primary siRNA indicated that non–RISC (RNA-induced silencing complex)–cleaved mRNAs are substrates for secondary siRNA production, infer that RdRPs perform unprimed RNA synthesis. Expand
A Distinct Small RNA Pathway Silences Selfish Genetic Elements in the Germline
The data suggest that rasiRNAs protect the fly germline through a silencing mechanism distinct from both the miRNA and RNA interference pathways. Expand
A Two-Hit Trigger for siRNA Biogenesis in Plants
Two "hits,"--often, but not always, two cleavage events--constitute a conserved trigger for siRNA biogenesis, a finding with implications for recognition and silencing of aberrant RNA. Expand
Analysis of the C. elegans Argonaute Family Reveals that Distinct Argonautes Act Sequentially during RNAi
Analysis of single- and multiple-AGO mutant strains reveals functions in several pathways, including chromosome segregation, fertility, and at least two separate steps in the RNAi pathway, supporting a two-step model for RNAi. Expand
Gene expression: Long-term gene silencing by RNAi
It is shown that a single episode of RNAi in the nematode Caenorhabditis elegans can induce transcriptional silencing effects that are inherited indefinitely in the absence of the original trigger. Expand
Large-Scale Sequencing Reveals 21U-RNAs and Additional MicroRNAs and Endogenous siRNAs in C. elegans
Another 18 microRNA (miRNA) genes were identified, thereby extending to 112 the authors' tally of confidently identified miRNA genes in C. elegans, and a third class of nematode small RNAs, called 21U-RNAs, was discovered. Expand
Regulation of AUXIN RESPONSE FACTOR3 by TAS3 ta-siRNA Affects Developmental Timing and Patterning in Arabidopsis
It is shown that the juvenile-to-adult phase transition is normally suppressed by TAS3 ta-siRNAs, in an ARGONAUTE7-dependent manner, through negative regulation of ARF3 mRNA. Expand
Systemic Spread of Sequence-Specific Transgene RNA Degradation in Plants Is Initiated by Localized Introduction of Ectopic Promoterless DNA
Systemic, posttranscriptional silencing of transgenes in Nicotiana benthamiana was initiated in localized regions of the plant by introduction of transgene-homologous DNA fragments, including thoseExpand