Amplification of the c-erbB-2 gene detected by FISH in gastric cancers.

Abstract

The amplification and overexpression of the c-erbB-2 gene are considered to be implicated in the process of carcinogenesis of a variety of human tumors. The amplification and overexpression of c-erbB-2 were investigated in 48 surgically resected human gastric cancers by means of fluorescence in situ hybridization and immunohistochemistry. DNA ploidy was determined by flow cytometry. The c-erbB-2 amplification was demonstrated as a cluster of signals, suggesting homogeneously staining region (HSR), in three tumors (6.3%) accompanied by the overexpression of its protein. Such overexpression was detected in another tumor without amplification of the c-erbB-2 gene. All tumors with amplification and overexpression of c-erbB-2 were differentiated adenocarcinoma histologically, but only 10.3 and 13.8% of differentiated carcinomas showed amplification and over-expression of the c-erbB-2 gene, respectively. There was no relationship between the amplification and overexpression of c-erbB-2 and the depth of tumor invasion and lymph node involvement. Three of four cases with overexpression of c-erbB-2 were classified into DNA aneuploid tumor.

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@article{Sato1997AmplificationOT, title={Amplification of the c-erbB-2 gene detected by FISH in gastric cancers.}, author={Tadashi Sato and Kazuho Abe and Akira Kurose and Noriko Uesugi and Takeshi Todoroki and K. Sasaki}, journal={Pathology international}, year={1997}, volume={47 2-3}, pages={179-82} }