Amplification of oak DNA from ancient and modern wood

  title={Amplification of oak DNA from ancient and modern wood},
  author={S. Dumolin-Lapegue and M. H. Pemonge and Ludovic Gielly and Pierre Taberlet and R{\'e}my J. Petit},
  journal={Molecular Ecology},
A polymorphic noncoding region of chloroplast DNA (cpDNA) was successfully amplified by the polymerase chain reaction (PCR) from various oak wood samples, including recent and more ancient (about 600‐years‐old) samples from different oak species. Adaptation of DNA isolation and amplification protocols was necessary to obtain this result. Polymorphisms useful to distinguish species or geographical origin of these samples could be scored through sequencing. These polymorphisms include one… 

Use of chloroplast microsatellites to differentiate oak populations

The results indicate that chloroplast microsatellites can be used for haplotype discrimination in many contexts including certification or traceability of oak material.

A set of primers for the amplification of chloroplast microsatellites in Quercus

This work has identified 17 potential cpSSRs motifs from available oak sequences and tested their variability among French oak populations, finding six loci were polymorphic at the intraspecific level in Quercus petraea and Q. robur.

Extraction, amplification and characterization of wood DNA from dipterocarpaceae

A successful DNA extraction from wood yielding appropriate DNA quality for PCR amplification allows molecular genetic investigations of wood tissue and potential applications are in forensics and in the control of the timber and wood trade.

Wood identification with PCR targeting noncoding chloroplast DNA

Five universal primer pairs amplifying chloroplast noncoding sequences of 300–1,200 bp were designed and successfully applied to extract DNA from the recalcitrant processed white oak wood and randomly selected staves of wine barrels, showing correlation to their geographical origins.

DNA from processed and unprocessed wood: factors influencing the isolation success.

Improved recovery of ancient DNA from subfossil wood - application to the world's oldest Late Glacial pine forest.

Stringent decontamination of wood surfaces combined with DNA metabarcoding and assessment of post-mortem DNA damage allowed us to authenticate ancient DNA retrieved from the oldest Late Glacial pine forest.

Wood identification of JapaneseCyclobalanopsis species (Fagaceae) based on DNA polymorphism of the intergenic spacer betweentrnT andtrnL 5′ exon

The present findings support the possibility of wood identification based on DNA polymorphism and distinguish Q. gilva from the other Quercus species, and the others are separated in two subgroups based onDNA polymorphism.



Association between chloroplast and mitochondrial lineages in oaks.

It is concluded that paternal leakage is not a significant factor at this timescale in many populations that are polymorphic for both cpDNA and mtDNA in this area without producing any new combination of cytoplasms involving the mitochondrial substitution.

Phylogeographic structure of white oaks throughout the European continent.

The mapped distribution of the haplotypes indicates the probable routes of postglacial recolonization followed by oak populations that had persisted in southern refugia, especially in the Iberian peninsula, Italy and the Balkans.

Polymorphic simple sequence repeat regions in chloroplast genomes: applications to the population genetics of pines.

It is anticipated that SSR loci within the chloroplast genome should provide a highly informative assay for the analysis of the genetic structure of plant populations, by using a PCR-based assay.

A set of universal primers for amplification of polymorphic non‐coding regions of mitochondrial and chloroplast DNA in plants

The genetic information present in the plant mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA) is of great interest in phylogeny and in population genetics, largely because of the non-mendelian

Chloroplast DNA footprints of postglacial recolonization by oaks.

Local systematic interspecific sharing of the maternal genome strongly suggests that long-distance seed dispersal events followed by interspecific exchanges were involved at the time of colonization, about 10,000 years ago.

Relationship Between Geographical Origin and Chemical Composition of Wood for Oak Barrels

Eight oak wood samples from the U.S., together with 39 samples coming from six different French regions, have been classified according to geographical origin. Extracts of the wood samples have been