Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV.

@article{Shoya1997AmplificationOA,
  title={Amplification of a full-length Borna disease virus (BDV) cDNA from total RNA of cells persistently infected with BDV.},
  author={Y Shoya and Takeshi Kobayashi and Toshiaki Koda and Patrick K Lai and Hiroyuki Tanaka and Tomohiro Koyama and Kazufumi Ikuta and Mitsuaki Kakinuma and Masahiko Kishi},
  journal={Microbiology and immunology},
  year={1997},
  volume={41 6},
  pages={481-6}
}
We have developed a novel reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify the full-length 8.9 kilobase (kbp) cDNA of the Borna disease virus (BDV) RNA genome from the total cellular RNA of MDCK cells persistently infected with BDV (MDCK/BDV). Antigenomic BDV cDNA was reverse transcribed using a 53-mer oligonucleotide primer, corresponding to the 5'-terminus of a putative 3'-leader sequence of the BDV RNA genome, for 2 hr at 42 C followed by 30 min at 55 C. PCR was performed… CONTINUE READING