Amplification of a Chimeric Bacillus Gene in Chloroplasts Leads to an Extraordinary Level of an Insecticidal Protein in Tobacco

@article{McBride1995AmplificationOA,
  title={Amplification of a Chimeric Bacillus Gene in Chloroplasts Leads to an Extraordinary Level of an Insecticidal Protein in Tobacco},
  author={Kevin E. McBride and Zora Svab and David J. Schaaf and Patric S. Hogan and David M. Stalker and Pal Maliga},
  journal={Bio/Technology},
  year={1995},
  volume={13},
  pages={362-365}
}
The Bacillus thuringiensis (Bt) crystal toxins are safe biological insecticides, but have short persistence and are poorly effective against pests that feed inside plant tissues. Production of effective levels of these proteins in plants has required resynthesis of the genes encoding them.We report that amplification of an unmodified crylA(c) coding sequence in chloroplasts up to ∼ 10,000 copies per cell resulted in the accumulation of an unprecedented 3–5% of the soluble protein hi tobacco… CONTINUE READING

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