Amplification of FISH signals using intermittent microwave irradiation for analysis of chromosomal instability in gastric cancer.

@article{Kitayama1999AmplificationOF,
  title={Amplification of FISH signals using intermittent microwave irradiation for analysis of chromosomal instability in gastric cancer.},
  author={Yasuhiko Kitayama and Hisaki Igarashi and Haruhiko Sugimura},
  journal={Molecular pathology : MP},
  year={1999},
  volume={52 6},
  pages={357-9}
}
Gastrointestinal tract tumours are notorious for their difficulty in relation to conventional cytogenetic analysis. In particular, necrosis, the presence of stromal inflammatory and other cells, and poor attachment of tumour cells have led to problems with the quality and reliability of cytogenetic preparations, even with the recently developed fluorescence in situ hybridisation (FISH) technique. Furthermore, background autofluorescence masks the weak hybridisation signals in the nuclei. To… CONTINUE READING