Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites.

@article{Eakin1990AmplificationAS,
  title={Amplification and sequencing of genomic DNA fragments encoding cysteine proteases from protozoan parasites.},
  author={Ann E. Eakin and Jean Bouvier and Judy A. Sakanari and Charles S. Craik and James H McKerrow},
  journal={Molecular and biochemical parasitology},
  year={1990},
  volume={39 1},
  pages={1-8}
}
Cysteine protease gene fragments from three protozoan parasites Trypanosoma cruzi, Trypanosoma brucei, and Entamoeba histolytica were amplified by the polymerase chain reaction (PCR) from genomic DNA using degenerate oligonucleotide primers. The primers used for the amplification were designed based upon amino acid sequences flanking the active site cysteine and asparagine residues that are conserved in the eukaryotic cysteine proteases analyzed to date. The amplified DNA fragments… CONTINUE READING