Amplification and sequencing of DNA from a 120–135-million-year-old weevil

@article{Cano1993AmplificationAS,
  title={Amplification and sequencing of DNA from a 120–135-million-year-old weevil},
  author={Ra{\'u}l J. Cano and H. N. Poinar and Norman J. Pieniązek and Aftim Acra and George Poinar},
  journal={Nature},
  year={1993},
  volume={363},
  pages={536-538}
}
DNA has been successfully isolated from both fossilized plant1 and animal tissues2–6. The oldest material, dated as 25–40 million years old (Tertiary), was obtained from amber-entombed bees4,5 and termites6. Tissues from both these insects yielded DNA of good quality, which could be amplified by the polymerase chain reaction (PCR) and subsequently sequenced, including the genes encoding 18S ribosomal RNA5,6 and 16S rRNA6. We report here the extraction of DNA from a 120–135-million-year-old… 

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References

SHOWING 1-10 OF 13 REFERENCES

Chloroplast DNA sequence from a Miocene Magnolia species

TLDR
The extraction of DNA from fossil leaf samples from the Miocene Clarkia deposit, the amplification of an 820-base pair DNA fragment from the chloroplast gene rbcL from a fossil of the genus Magnolia, and its subsequent sequencing extend the ability to analyse ancient DNA and may open new avenues into problems in palaeobotany, biogeography, and in the calibration of mutation rates.

DNA sequences from the quagga, an extinct member of the horse family

TLDR
Dried muscle from a museum specimen of the quagga, a zebra-like species that became extinct in 1883, is examined, and DNA was extracted in amounts approaching 1% of that expected from fresh muscle, and that the DNA was of relatively low molecular weight.

DNA sequences from a fossil termite in Oligo-Miocene amber and their phylogenetic implications.

TLDR
Phylogenetic analysis of fossil and extant 18S rDNA confirmed morphological cladistic analyses of living dictyopterans (termites, cockroaches, and mantids) and affects molecular phylogenetic hypotheses of termites in this, the oldest DNA yet characterized.

DNA phylogeny of the extinct marsupial wolf

TLDR
The genetic data suggest that the Marsupial wolf was more closely related to other Australian marsupial carnivores than to those of South America, and represents an example of convergent morphological evolution to South American car-nivorous marsupials as well as to true wolves.

The number of replications needed for accurate estimation of the bootstrap P value in phylogenetic studies.

  • S. Hedges
  • Biology
    Molecular biology and evolution
  • 1992
TLDR
The purpose of the present report is to explore the variance (and hence the accuracy) of the phylogenetic BP and to establish guidelines for efficient bootstrap sampling.

The neighbor-joining method: a new method for reconstructing phylogenetic trees.

TLDR
The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.