Amplification and sequencing of DNA from a 120–135-million-year-old weevil

  title={Amplification and sequencing of DNA from a 120–135-million-year-old weevil},
  author={Ra{\'u}l J. Cano and H. N. Poinar and Norman J. Pieniązek and Aftim Acra and George Poinar},
DNA has been successfully isolated from both fossilized plant1 and animal tissues2–6. The oldest material, dated as 25–40 million years old (Tertiary), was obtained from amber-entombed bees4,5 and termites6. Tissues from both these insects yielded DNA of good quality, which could be amplified by the polymerase chain reaction (PCR) and subsequently sequenced, including the genes encoding 18S ribosomal RNA5,6 and 16S rRNA6. We report here the extraction of DNA from a 120–135-million-year-old… 

Phylogenetic Utility of Partial Dna Sequences of G6pdh at Different Taxonomic Levels in Hexapoda with Emphasis on Diptera

The utility for insect systematics of an ≈480-bp fragment of the coding region of the gene G6pdh, which encodes the pentose phosphate shunt enzyme glucose-6-phosphate dehydrogenase, is evaluated.

The use of herbarium specimens in DNA phylogenetics: Evaluation and improvement

Experiments are described about trials of DNA amplification from two to 151-year-old herbarium specimens of plant species the authors needed for their projects, and successful amplification was obtained in eight samples out of the 17 examined.

Absence of Ancient DNA in Sub-Fossil Insect Inclusions Preserved in ‘Anthropocene’ Colombian Copal

It is concluded that DNA is not preserved in insects preserved in copal, raising further doubts about claims of DNA extraction from fossil insects in amber, many millions of years older than copal.

Little Evidence for the Preservation of a Single-Copy Gene in Charred Archaeological Wheat

DNA extracts from charred archaeological cereal grains from the Northern Alpine region screened for the presence of authentic DNA by PCR amplification of a 240 bp fragment from the high molecular weight glutenin subunit gene promoter (HGP) region found most positive results of the HGP regions were subsequently considered not authentic.

Museum fish specimens and molecular taxonomy: A comparative study on DNA extraction protocols and preservation techniques

The results show that mtDNA extraction, amplification and sequencing of conserved genes could be obtained successfully from frozen (−20°C) preserved specimens and also from ethanol- preserved specimens but not from any of the formalin (10%) fixed specimens (3–4 years).

Effects of preservation methods, parasites, and gut contents of black flies (Diptera: Simuliidae) on polymerase chain reaction products.

Sufficient DNA was extracted from the head of a larva for a successful polymerase chain reaction (PCR), eliminating the need to remove the contaminating gut and parasites.

Ancient DNA from amber fossil bees?

This work focused on the Dominican amber fossil stingless bee Proplebeia dominicana, and began by obtaining partial sequences of mariner (Robertson et al. 1997) and Tel (Avancini, Walden, and Robertson 1996) family transposons from four extant relatives (see below).


Morphological, allozyme, and chromosomal characters and ecological traits have limited value for discriminating among four closely related Pissodes spp.

Recovery and analysis of ancient beetle DNA from subfossil packrat middens using high-throughput sequencing

The results of the first targeted investigation of insect ancient DNA to positively identify subfossil insects to species are reported, which includes the recovery of endogenous content from samples as old as ~ 34,355 ybp.

Bacillus DNA in fossil bees: an ancient symbiosis?

The close phylogenetic association of the extracted DNA sequences with these bee colonizers suggests that a similar bee-Bacillus association existed in the extinct species P. dominicana.



Chloroplast DNA sequence from a Miocene Magnolia species

The extraction of DNA from fossil leaf samples from the Miocene Clarkia deposit, the amplification of an 820-base pair DNA fragment from the chloroplast gene rbcL from a fossil of the genus Magnolia, and its subsequent sequencing extend the ability to analyse ancient DNA and may open new avenues into problems in palaeobotany, biogeography, and in the calibration of mutation rates.

DNA sequences from the quagga, an extinct member of the horse family

Dried muscle from a museum specimen of the quagga, a zebra-like species that became extinct in 1883, is examined, and DNA was extracted in amounts approaching 1% of that expected from fresh muscle, and that the DNA was of relatively low molecular weight.

DNA sequences from a fossil termite in Oligo-Miocene amber and their phylogenetic implications.

Phylogenetic analysis of fossil and extant 18S rDNA confirmed morphological cladistic analyses of living dictyopterans (termites, cockroaches, and mantids) and affects molecular phylogenetic hypotheses of termites in this, the oldest DNA yet characterized.

DNA phylogeny of the extinct marsupial wolf

The genetic data suggest that the Marsupial wolf was more closely related to other Australian marsupial carnivores than to those of South America, and represents an example of convergent morphological evolution to South American car-nivorous marsupials as well as to true wolves.

The number of replications needed for accurate estimation of the bootstrap P value in phylogenetic studies.

  • S. Hedges
  • Biology
    Molecular biology and evolution
  • 1992
The purpose of the present report is to explore the variance (and hence the accuracy) of the phylogenetic BP and to establish guidelines for efficient bootstrap sampling.

The neighbor-joining method: a new method for reconstructing phylogenetic trees.

The neighbor-joining method and Sattath and Tversky's method are shown to be generally better than the other methods for reconstructing phylogenetic trees from evolutionary distance data.